Nominal volume: 30L

Diameter to height ratio: 1:2.5Liquid loading coefficient:70%

30Tank body: The main material is all stainless steel316LTank body, with no dead corners inside; Equipped with dedicated sampling and discharging valves for fermentation tanks, as well as transfer pipelines. Using a wide-angle liquid level observation mirror inside the tank,12VSafety sight light with temperaturePH、DODefoaming, liquid level interface, inoculation port, feeding port And multiple spare ports, all welds are sturdy, neat and beautiful.The pressure gauges in contact with the material are all diaphragm pressure gauges equipped with safety rupture discs, Tank Bottom Diaphragm Valve Bailun

Tank body: design pressure0.3Mpajacket : Design pressure0.3MpaBy adopting optimized diversion design, the exchange efficiency and uniformity of temperature inside the tank have been improved. Stable and uniform temperature control is the basic condition for successful fermentation.

Tank function:Upper mixing mechanical seal, intake sterilization filter, exhaust condenser, intake system with air distributor, flame inoculation port (also feeding port and cleaning port), discharge port, sampling port, and replenishment port; The electrodes for automatic control parameters are:PHElectrodeDOElectrode, liquid level electrode, defoaming electrode, temperature electrode port; Four peristaltic pumps are automatically controlled to replenish acid separately/alkali/GPe/Cultivation agent;

Motor: exclusively for fermentation tanksSEWCommunicate the motor to ensure that it can operate in harsh environments;

Ventilation: Adopting a deep ventilation system, manually controlled by a rotor flowmeter, adjusting the gas flow rate according to process requirements, and evenly distributing gas through an air distributor inside the tank; Adopting an accuracy of0.01μmThe sterilization filter is equipped with an anti backflow device to ensure safety and greatly extend its service life;

stirstir and mixsystem:Mechanical coupling mixing system, John Crane single end mechanical seal, using316LStainless steel special stirring shaft material, precision machined, with ideal dynamic balance performance, good rigidity, and long-term use without deformation; The entire system is safe, stable, and has a long lifespan;

Mixing form:Straight blade agitator (second stage), intermediate inclined blade agitator, mechanical compression defoamer (first stage), four baffle plates;

Fermentation pH control function: adopting intelligent technologyPIDControl, dedicated to Mettler SwitzerlandPHElectrode;

Fermentation dissolved oxygen control function: adopting intelligent technologyPIDControl, dedicated to Mettler SwitzerlandDOElectrode;

Temperature control: automatic heating, water cooling, circulation pump circulation temperature control, with air self escape function and water cut-off, over temperature protection function, with good heat exchange efficiency; The intelligent module control ensures both the speed of temperature control and energy saving.

Feeding: A comprehensive feeding system (acid, alkali, foam, culture agent) that can provide timed and quantitative feeding; Four peristaltic pumps can be selected for segmented settings on the control interface, which is convenient and flexible in practical use. Equipped with specially designed feeding pins to ensure safe operation of feeding;

Sterilization:In situ automatic sterilization (oil bath electric heating)

Vaccination:Alcohol flame inoculation+Needle insertion method for vaccination;

The above functions basically meet the requirements of laboratory and various conventional fermentation experiments;

2Technical parameters

Mixing speed: using fermentation tank specificSEWAC motor, wide range, low noise, high temperature resistance, long life, constant torque;

Motor Power30rise380W; Speed: fifty~1000rpmContinuously variable transmission; Accuracy: ±10rpm;

PHControl:PHElectrode, using intelligent technologyPIDControl, peristaltic pump automatically replenishes acid/Alkali; It can make the control performance of acidity and alkalinity very excellent; range: two~12ph;Control accuracy:±0.02ph,resolution: 0.01ph；

DOControl:DOElectrode, using intelligent technologyPIDControl, can be associated with speed; The control of dissolved oxygen parameters has achieved ideal results; Range:0~100%Control accuracy:±3%;resolution: 0.1%；

Temperature control:PT100Electrode detection, cascade automatic control; Electric heating, water cooling; The Youbei intelligent module control ensures the speed and accuracy of temperature control; range:Cooling water temperature+5~60℃； accuracy:±0.1℃； resolution: zero point one℃；

Foam control: electrode detection, peristaltic pump replenishing defoamer, automatic control;

Ventilation: Manual control of rotor flowmeter

Tank pressure: manual adjustment of diaphragm valve, digital display of pressure gauge and pressure electrode; Electromagnetic valve jog control pressure, range: 0~0.20Map；

3Control system

Siemens GermanyS7-200seriesPLCcontrol system+Embedded industrial control computer touch screen display and operation

BLBIO BType control system: on-site controller, with Siemens as the lower computerS7-200seriesPLCcontrol system,It is a mature, stable, and universal typePLCIt can be suitable for various applications of automation, especially in the control of production and manufacturing processes. Its modularity, ease of implementing distributed configuration, and ease of mastery are the characteristics of SiemensPLCThe control core has become an economical and advanced control system for implementing biological reaction process control,The system adopts touch screen display and on-site direct operation (menu style), with fully Chinese menus and interfaces; It can also transmit data with the upper computer, sample and display tank data, browse and analyze system data, and read settings.

use10The inch integrated LCD touch screen serves as the display interface, with rich and complete display content,Humanized graphics are simple and clear, with easy and convenient operation. And they don't interfere with each other. This control system can adapt to the output and input signals of different sensors and actuators, and is equipped with a signal anti-interference system.

Control and display parameters: temperature, stirring speedpHDissolved oxygen, defoaming, air flow rate, tank pressure, fermentation broth volume, exhaustO2Exhaust gasCO2Wait, wait14It has direct parameters and can calculate indirect parameters.

1Control method

① Manual control mode: valve opening ratio or valve opening degree can be set. ② Automatic control mode: optionalPIDContinuous orPIDSwitching mode control ③ Sequential control mode: All control parameters can be pre-set at least10A control segment is used to achieve automatic segmented control. ④ Remote control mode: can accept upper computer control. ⑤ Associated control: dissolved oxygen can be controlled by selecting speed, air flow rate, tank pressure, feeding, etc;pHYou can choose to add acid and alkali to control it

Measurement function: It can measure replenishment, foaming, acid and alkali, etc

X YThe axis moving diagram can display and expand the graph freely, helping operators to determine the mutual influence between parameters and quickly modify and adjust the fermentation process

Data processing function

Capable of displaying and analyzing trend curves for all parameters

Can store, display, and analyze historical curves of all parameters

Batch number report that can display all parameters

Can directly copy data with a USB drive

Data storage format andEXCELCompatible, available up toEXCELPlatform processing

Controller self-protection function: password can be set, others cannot modify fermentation parameters; And the controller does not lose the set values of each parameter due to power failure

Capable of program control over various parameters,

The time display record interval for each parameter can be adjusted arbitrarily

Can set and calibrate two parameters on the same screen

9.The anti false operation status displayed on the controller, the panel status flow indication system, the process hardware and action status display lights are used to understand the working status of various related components (such as peristaltic pumps, circulation pumps, heating, stirring, etc.), and can be used as fault discrimination displays

10Each parameter of the transmission is an independent module that does not interfere with each other and is easy to maintain

11.PH、DOThe parameter control method and associated control program can be adjusted freely

Computer software:BLBIO-Ver8.0It is a fermentation specific software package that integrates data recording and processing, with a Chinese operating interface, data storage backup, curve analysis, report generation analysis, anomaly analysis, etc. It brings a lot of convenience to the monitoring and process data processing of fermentation experiments and production.

Main functions:

Can record fermentation time, temperaturepHDissolved oxygen, rotational speed, air flow rate, pressure, feeding amount, foaming amount, acid-base dosage, etc

Can send the set values and control parameters of self-control variables,Implement remote control operation

Can set sequence control

You can input offline parameters,And record and display it

Trend curve and historical curve display

Can display simultaneously1-10Curve (arbitrarily set);

You can choose different starting times and time periods（8、24、48、72、96 、144Hours);

You can freely set the upper and lower limits of the curve display,To improve display resolution;

You can choose any number of different batch numbers（1-10Display the curves simultaneously for comparative analysis;

giveYThe parallel reading tangent line of the axis can be dragged by the mouse,Subsequently, the curve data is activated,Fermentation time(Xaxle)And at this time, the data of the curve changes simultaneously with the movement of the secant;

The software screen can display all parameter curves on the same interface, and can also be set to display one or several parameter curves and set the range

The parameter curve can achieve time gradients before and after fermentation (the length range of the time coordinate axis can be from10Minutes to arrive10Choose freely within the day (pan, zoom in)

You can choose to display and zoom in on online or historical parameter curves for different time periods, batches of data, and curves

You can combine the seed tank and fermentation tank on the same screen2Compare the parameter curves at any time period

One click switching between real-time and historical curves, restoration of real-time curves, and the ability to set and modify curves“style”，“colour”，“variable”，“lower limit”reach“high limit”

PRINT

Can display simultaneously1-12Individual (arbitrarily set) data,And it can be printed and output;

You can choose different starting times and time periods,And different time intervals can be selected(1，5，15，30，60minute)；

You can choose any number of data from different batch numbers (set arbitrarily),And it can be printed out,

For comparative analysis;

Curve printing

The displayed curve can be printed out using a color printer;

Can also be found in theEXCELData processing and printing on the platform,Very convenient.

The Bailun Huishen analysis software (to be purchased separately) collects environmental parameters, operating parameters, cell metabolism parameters, and other data from the control system of the fermentation tank into this system, and stores them in batches. The experimental process is displayed to process analysts in the form of graphics or tables. Various data derived from offline analysis and formula calculations can be collected into a batch data report. Can compare multiple batches of data.

1Accurate calculation onlineOUR、CER、RQ、KLaBy using indirect parameters, multiple curves with different parameters can be displayed simultaneously on the same coordinate plane; Convenient for users to analyze the correlation of the fermentation process, in order to identify appropriate control points and achieve the goal of one-step scaling up in pilot testing;

2Can input offline detection data such as total sugar, reducing sugar, amino nitrogen, bacterial concentration, fermentation unit, etc. and generate corresponding curves under online process parameter curves;

1

Respiratory filter

0.2μm， φ37

6 pieces

Germany

2

intake filter

0.2μm φ50

6 pieces

Shanghai Yiming or Dominic

3

Supplement bottle

500ml

2 pieces

Shanghai

4

Supplement bottle

1000ml

2 pieces

Shanghai

5

Two port interface for replenishment bottle

general

12 pieces

Shanghai Bailun

6

PH standard solution

general

1 set

Shanghai

7

Single hole replenishment pin

general

12 sets

Shanghai Bailun

8

Silicone stopper

general

30 pieces

Shanghai Bailun

9

silicone hose

16#

5 meters

France

10

silicone hose

25#

5 meters

France

11

o-ring

general

20 of them

Germany

12

fuse

5A/10A

15 of them

Shanghai

13

tool

general

1 set

Shanghai

14

Equipment Operation Manual

1 set

Shanghai Bailun

15

Product Qualification Certificate

1 set

Shanghai Bailun

16

Acceptance Form

1 set

Shanghai Bailun