Detection of Alcohol Content in Blood by Headspace Gas Chromatography

Blood alcohol analysis chromatographsummary

The frequent occurrence of drunk driving and the increasing number of traffic accidents caused by drunk driving have made it an important and arduous task for judicial departments in various regions to quickly, accurately, and stably analyze the alcohol content in the blood of drunk driving drivers. The gas chromatograph produced by our company is connected to the headspace sampler for use together, formingBlood alcohol analysis chromatographInstrument. The detection of alcohol content in blood is based on the analysis requirements and conditions specified in the public safety industry standard GA/T842-2009 of the People's Republic of China, which can quickly and accurately analyze the alcohol content in human blood. Can meet the needs of judicial departments across the country. It has successfully passed the acceptance of the * system judicial appraisal center.

Mainly used in: * bureaus * teams, major hospitals, judicial appraisal centers, etc.

Instrument configuration:

GC-7890/7900/9860 gas chromatograph with hydrogen flame detector

2. HS-9A/160/100/200 headspace sampler

3. Chromatographic column for alcohol analysis in blood

4. Nitrogen, hydrogen, and air source gases with a purity of 99.999%

5. Data processing system (computer, printer, chromatography workstation)

6. Glassware: 2 10mL graduated straws,

6 1mL and 100mL volumetric flasks each,

One 500uL pipette (with disposable pipette head),

2 1mL graduated straws,

A test tube coated with anticoagulant is like one

7. Reagent: Anhydrous ethanol (analytical grade)

Tert butanol (analytical grade)

N-propanol (analytical grade)

distilled water

Working principle and advantages:

Automatically heat the sample according to the set value after startup; Needle insertion; Sampling; Fill the sample into the quantitative tube; Six way valve switching injection; Start the workstation to start sampling and recording work
Analysis is to determine the content of these components in the original sample by analyzing the gas composition above the sample matrix Obviously, this is an indirect analysis method, and its basic theoretical basis is that there exists a distribution equilibrium between the gas phase and the condensed phase (liquid phase and solid phase) under certain conditions So, the composition of the gas phase can reflect the composition of the condensed phase We can regard headspace analysis as a gas-phase extraction method, which uses gas as a "solvent" to extract volatile components from samples. Therefore, headspace analysis is an ideal sample purification method Traditional liquid-liquid extraction and SPE involve dissolving the sample in a liquid, inevitably leading to some co extracted substances interfering with the analysis Moreover, the purity of the solvent itself is also an issue, which is particularly important in trace analysis Using gas as a solvent can avoid unnecessary interference, as high-purity gases are easily obtainable and cost-effective, which is also an important reason why headspace GC is widely used
As an analytical method, headspace analysis is first and foremost simple, as it only takes the gas phase for analysis, greatly reducing the interference of the sample matrix on the analysis As a sample processing method for GC analysis, headspace is the most convenient Secondly, headspace analysis has different modes that can be adapted to various samples by optimizing operating parameters Thirdly, the sensitivity of headspace analysis can meet regulatory requirements. After that, combined with the quantitative analysis ability of GC, headspace GC * can perform accurate quantitative analysis

4、 Application scope of headspace gas chromatography:
(1) Forensic Science: Testing of volatile toxins such as alcohols, aldehydes, and ketones in blood and urine
(2) Traffic Safety: Testing of Blood Alcohol Content for Vehicle Drivers Drinking and Drunk Driving
(3) Brewing industry: determination of low boiling point aroma components in beer
(4) Food industry: detection of residual 6 # solvent in extracted oil
(5) Environmental protection: Determination of volatile halogenated hydrocarbons in surface water and tap water such as rivers, lakes, etc
(6) Pharmaceutical industry: detection of residual solvents in drugs
(7) Material control: detection of residual acetaldehyde in beverage bottles

(8) Coatings and paints: detection of organic volatile compounds such as benzene, toluene, and xylene in coatings and paints

(9) Packaging printing: detection of residual organic solvents in flexible packaging bags