For optical imaging and electrophysiological experiments, isolating myocardial cells from perfused hearts is the first and most important step. The perfusion system can perform constant pressure or cross flow Langendorff perfusion. The experimenter decided to typically use cross flow perfusion separation, where a shunt tube can be used to select the appropriate solution and perfuse it into the heart through a peristaltic pump. Firstly, Kreb's solution is used to clean blood cells and stabilize the specimen with ice skates. next. A solution containing EGTA is used to remove residual calcium. By circulating a solution containing collagenase or digestive enzymes to perfuse the specimen, it is known that the heart has become soft, indicating that the substances that maintain the arrangement between cells have melted. Place partially digested tissue pieces into a shaker water bath and continuously perform forced dissolution treatment until myocardial cells are released. The entire experimental process requires switching the solution several times to select the most viable myocardial cells. In order to increase vitality, it is necessary to periodically change the calcium concentration of the incubation medium and return myocardial cells to their adapted ionic environment before use. If the experimenter decides to use constant pressure perfusion mode for isolation, the perfusion bottle needs to be elevated, and the shunt tube directly selects the solution, which is then infused into the heart through the gas-liquid valve. The valve under the heart chamber can be used to discharge the perfusion solution or to recycle the source solution through a pump.

There are two configurations to choose from, the simplified 120107 cardiomyocyte isolation system. Complex 120108 cardiomyocyte isolation system

120107 Simplified Cardiomyocyte Separation System

Note: This product is mainly used as an experimental instrument for scientific research. Non clinical medical equipment.