Radnoti Working Heart System
The Working Heart ex vivo cardiac perfusion experiment method involves the flow of perfusion fluid from the left atrial appendage into the left ventricle. The left ventricle contracts to overcome the resistance of the aorta and inject the fluid into the simulated aortic duct, allowing the heart to perform perfusion experiments under physiological conditions. In this device, left ventricular filling pressure (preload) and aortic column pressure (afterload) can be kept constant, and heart rate can also be controlled through an electrical pacing device. Therefore, the three can be controlled simultaneously as needed to observe the effect of drugs on myocardial contractility, or to control two of them and observe the relationship between myocardial contractility and the other. In addition, by measuring certain substances in coronary outflow fluid, the effects of drugs or other factors on cardiac biochemistry can be understood.

General configuration of ex vivo cardiac perfusion system:
Rat/guinea pig/rabbit ex vivo working heart perfusion system, mouse ex vivo working heart perfusion system, pig ex vivo working heart perfusion system

Langendorff ex vivo cardiac perfusion system
Under constant pressure conditions, a cardiac cannula is inserted into the aorta of an isolated animal heart, and retrograde perfusion (k-h) fluid is administered to the myocardium through the coronary artery. The perfusion fluid flows out from the right atrium, pulmonary artery, and vena cava through the coronary sinus, and this outflow volume is called coronary flow (CBF). At the same time, it can also record myocardial contractility. In addition, inserting a balloon into the left ventricle can measure left ventricular pressure (LVP), left ventricular end diastolic pressure (LVEDP), left ventricular pressure change rate (dp/dt), and heart rate (HR).

Constant pressure non circulating ex vivo cardiac perfusion system
LANGENDORFF constant voltage non cyclic experiment can be conducted.. The experiment can be in a state of pulsatility or spontaneous heartbeat.
It can measure left ventricular pressure, myocardial tension, coronary flow (collected at night of perfusion), electrocardiogram, perfusion pressure, temperature, and ions. The advantage of a constant pressure system is that the coronary arteries can automatically adjust flow according to the needs of myocardial tissue. Suitable for drug titration, ischemia, and reperfusion

Constant current circulation ex vivo cardiac perfusion system
LANGENDORFF constant current circulation perfusion experiment can be conducted. The experiment can be in a state of pulsatility or spontaneous heartbeat.
It can measure left ventricular pressure, myocardial tension, coronary resistance, electrocardiogram, perfusion pressure, temperature, and ions.
Coronary resistance can be conveniently measured through changes in pressure. By using multiple containers or infusion pumps, different solutions or drug concentrations can be applied to the heart. Because it is a constant flow method, drugs are more easily administered quantitatively to the heart, but the heart will not be able to automatically regulate coronary flow, so relatively constant pressure perfusion will produce more ischemia or excess defects. This system is more suitable for pharmacological dose-response studies.
Suitable for myocardial cell isolation, drug titration, circulation of valuable drugs, metabolism, ischemia and reperfusion, and release of endogenous mixtures.

Constant current non circulating ex vivo cardiac perfusion system
LANGENDORFF constant current non circulating perfusion experiment can be conducted. The experiment can be in a state of pulsatility or spontaneous heartbeat.
It can measure left ventricular pressure, myocardial tension, coronary resistance, electrocardiogram, perfusion pressure, temperature, and ions.
Coronary resistance can be conveniently measured through changes in pressure. By using multiple containers or infusion pumps, different solutions or drug concentrations can be applied to the heart. Because it is a constant flow drug that is more convenient to quantitatively apply to the heart, but the heart cannot automatically regulate coronary flow, so relatively constant pressure perfusion will produce more ischemia or excess defects. This system is more suitable for pharmacological dose-response studies.
Suitable for drug titration, ischemia, and reperfusion

*Please specify the type of animal to be made when placing an order, such as rats, cats, or guinea pigs. To configure appropriate cardiac chambers

Composition of ex vivo cardiac perfusion system

The structure of an ex vivo cardiac perfusion system varies depending on the perfusion mode. At the same time, the specific structure of the experimental system varies depending on the experimental subjects (such as rabbits, rats, mice). Based on the existing equipment of the customer, they can also selectively order some components in the system. Whether to choose a data collection system. The overall system is mainly divided into irrigation system and data acquisition and analysis system

Here are some common components of irrigation systems:

Data collection and analysis system
The measurement configuration can be selected according to the user's needs. Common measurement parameters include electrocardiogram, myocardial tension, left ventricular pressure, perfusion pressure, temperature, etc.

Other optional accessories

Note: This product is mainly used as an animal experimental instrument for basic medical research. Non clinical medical equipment.

Related products:

New integrated ex vivo cardiac perfusion system

Note: This product is mainly used as an experimental instrument for scientific research. Non clinical medical equipment.