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Varioskan Flash full wavelength multifunctional enzyme-linked immunosorbent assay (ELISA) readerProduct Introduction|thermoelectricity|Thermo Scientific： | | As a new generation grating type multifunctional microplate reader, Varioskan can provide various experimental settings parameters for pharmaceutical and biological research to achieve the most ideal experimental performance. Varioskan offers an extremely wide wavelength range, and optional splitters can optimize dynamic experiments and help achieve maximum sensitivity for fluorescence and absorption light detection. Moreover, the excellent usability and graphical interface of SkanIt software make it very easy to create experimental plans. |
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Product Features: | Detection type
Ø UV/visible/near-infrared light density test
Ø UV/Visible Fluorescence Density Test
UV/visible time-resolved fluorescence test
Ø Flash/glow chemiluminescence test
Endpoint method experiments, fast/slow kinetics experiments, and real-time liquid addition can be conducted in various types of experiments
Detection modes such as rapid kinetic experiments, spectral scanning, and pore scanning.
Continuous Spectral Detection - Optimizing Experimental Conditions
Ø Equipped with fluorescence continuous spectrum scanning function
Ø Equipped with optical density continuous spectral scanning function
Wide wavelength range - suitable for almost all experiments that quantify solutions through fluorescence/optical density spectroscopy
Simultaneous detection of multiple wavelengths - improves the parallelism of detection
Optional 3 liquid separators - ensuring the integrity of the dynamic curve and capable of detecting instantaneous luminescence experiments
Four grating design - improving spectral purity and ensuring experimental sensitivity
UV fluorescence detection - truly achieving UV fluorescence excitation/reception
Optical path correction technology - breaking through the bottleneck between enzyme-linked immunosorbent assay (ELISA) and spectrophotometer
Suitable for various board types - adaptable to different testing needs
Three level incubation system - providing the best detection temperature to avoid errors caused by evaporation
Adjustable oscillation function - improving the accuracy of heterogeneous solution detection
Multi angle optimization of experimental conditions - eliminating more uncertain factors for the reliability of experimental results
Skanlt software provides logical experimental program settings
application
Cytological detection GPCR assay
FRET experiment for Ca2+channel detection
Reported Gene Experiment Enzyme Dynamics Determination
Cell apoptosis ELISA/FIA/TRF-ELISA detection
Multi label detection of cell proliferation
Cytotoxicity and ADMETox ion channel experiments
Signal Transduction TR-FRET Experiment
Molecular interaction of tryptophan and tyrosine fluorescence
DNA, RNA, and protein quantification firefly luciferase luminescence assays |
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Product parameters: | Fluorescence spectrophotometer section (fluorescence and TRF):
Reading board type: 6-hole -1536 hole board
Wavelength selection: 4 monochromators
Excitation light wavelength: 200-1000nm
Emission wavelength: 270-840nm
Excitation light bandwidth: 5nm or 12nm
Emission bandwidth: 12nm
Wavelength resolution: 1nm
Light source: Xenon flash lamp
Detector: Photomultiplier Tube (PMT)
Sensitivity: Fluorescence top reading<0.4 fmol fluorescein/well (black 384 plate)
Bottom reading<4.0 fmol fluorescein/well (black 384 plate)
Time resolved fluorescence<120 amol fluorescein/well (white 384 plate)
Fluorescence detection range: Fluorescence>6 decks (black 384 plate)
TRF time-resolved fluorescence>6 decks (white 384 plate)
Measurement speed: 96 well plate, 15 seconds; 384 well plate, 45 seconds; 1536 orifice plate, 135 seconds (when selecting the minimum dynamic interval time)
Chemiluminescence part
Reading board type: 6-1536 orifice board
Wavelength selection: any wavelength, filter and dual monochromator
Wavelength range: High sensitivity mode: 360-670nm, scanning mode: 270-840nm
Sensitivity:< 7amol luciferase/well
Detector: Dark Current Photon Counter
Colorimeter section:
Reading plate type: 6-well to 384 well plate
Wavelength selection: 2 monochromators
Wavelength range: 200-1000nm
Bandwidth: 5nm
Wavelength resolution: 1nm
Light source: Xenon flash lamp
Detector: Photodiode (PDT)
Linear range: 0-4Abs, (96 well plate) at 450nm,<± 2%
0-3Abs, (384 well plate) at 450nm,<± 2%
Absorption value resolution: 0.001Abs
Accuracy:<± 1%, at 400-1000nm (0-3Abs)
< ±2%, at 200—399nm （0-2Abs）
Accuracy: SD<0.0001Abs or CV<0.5%, at 450nm (0-3Abs)
Measurement speed: 96 well plate, 15 seconds; 384 well plate, 45 seconds; 1536 orifice plate, 135 seconds (when selecting the minimum dynamic interval time)
Liquid separator
Applicable plate type: 6-384 well plate
Separation volume: 1-1000ml, incremental 1ml
0.40mm separating nozzle: accuracy:<0.2ml or 2%, 5-1000ml
Accuracy:<2% (20-1000ml); <5% (5-19ml)
0.25mm dispensing nozzle: accuracy:<0.2ml or 2%, 1-20ml
Accuracy:<10% (when 1-4ml); <5%, (5-20ml)
Separation speed: 30 seconds, 96 well plate, 5ml/well, 0.40mm separation nozzle
80 seconds, 384 well plate, 5ml/well, 0.40mm pipette tip
Incubator: Temperature range: Room temperature+4 ° C to 45 ° C
Oscillator: Circular oscillation, adjustable speed and diameter |
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