High performance liquid chromatography Primaide

Product Introduction:

High performance liquid chromatography (HPLC) is widely used in fields closely related to people's daily lives, such as medicine, food, chemistry, and environment, and is the most widely used analytical instrument. Hitachi's Primaide high-performance liquid chromatograph pursues reliability and durability, ensuring that you can use it stably for a long time and providing strong support for your daily analytical work. We recommend the Hitachi HPLC product Primaide, which has over 50 years of experience in HPLC research and production. Primaide features a simple design, exquisite and stylish appearance with built-in optional accessories, and easy maintenance of components. It is an easy-to-use HPLC product.

System features:

1. Creating a refined and stylish system appearance through the built-in selection of accessories

The main optional components such as degassing device, gradient unit, and sample cooling unit are all built into the host, and even if optional components are added, they do not occupy additional space.

2. Improve operability and maintainability through component pre installation

All modules can be operated from the front, and piping is also concentrated on the front, making it easy to replace chromatography columns and ensure smooth operation of the flow system. Considering maintenance, it is possible to replace lights or circulation pools from the front.

3. All modules are equipped with leakage sensors

When there is a leakage in the flow system, the leakage sensors configured in each module can sense and automatically alarm, improving safety and reliability.

4. Control through system management software

All modules can be controlled through system management software without the need for an operation panel.

5. Communication using E-Line and USB interfaces

Compared to traditional D-Line communication, it is more advanced in interconnecting various modules, centralizing all communication, digitizing signals, and improving the reliability of traffic. The connection with the PC adopts a highly universal USB interface, and the PC does not require any special boards.

6. GLP function

Each detector is equipped with a built-in mercury lamp, which uses its characteristic spectral line (254um) to automatically calibrate the wavelength in the ultraviolet region. Each module has quality assurance and high data reliability.

The application of Primaide in various fields

1. Food

1) Determination of organic acids

Organic acids are acidic organic compounds containing carboxyl groups (excluding amino acids), which are the main flavor nutrients in fruits. They can soften blood vessels, enhance appetite, aid digestion and absorption, and quench thirst and heat.

2) Determination of water-soluble vitamins

Vitamins are a type of organic substance essential for maintaining human life activities, which can be divided into water-soluble vitamins and fat soluble vitamins. This example involves the simultaneous analysis of 9 water-soluble vitamins using a reverse phase chromatography column and detection using DAD, while obtaining better wavelength chromatograms for different substances. The use of DAD detector can obtain spectral and peak purity information, which is particularly effective for samples with complex components such as food.

2. Drugs

Determination of medicinal materials

Compounds formed by connecting with another type of non sugar substance are the active ingredients of medicinal herbs.

The non sugar portion (glycosidic ligand) has UV absorption and can be analyzed using HPLC. In addition, the use of DAD detector can also be compared with the absorption spectrum of standard reference materials to qualitatively identify peaks detected in medicinal materials other than glycosides.

3. Biology

Peptide mapping method

Protein is digested using chemical methods or enzymes, and the resulting peptide fragments are separated and detected using HPLC. By comparing their chromatographic patterns, changes in the amino acids that make up the protein are confirmed.

To confirm the stability of the method, multiple LC chromatograms with peptide peaks were compared, and the reproducibility of retention time and peak area was good.

Analysis of catechins

[Standard Sample]

GC: (-) - Epicatechin gallate (thermal isomer) EGC: (-) - Epigallocatechin gallate C: (-) - Catechins EGCG: (-) - Epigallocatechin gallate EC: (-) - Epicatechins GCG: (-) - Epicatechin gallate (thermal isomer) ECG: (-) - Epicatechin gallate CG: (-) - Epicatechin gallate (thermal isomer) Caffeine ： Coffee because