LCpro T Fully automatic portable photosynthetic apparatus

foreword

LCpro-TPortable photosynthesis meter is a new generation of intelligent portable photosynthesis measuring instrument, used to measure parameters related to plant photosynthesis such as photosynthetic rate, transpiration rate, stomatal conductance, etc. of plant leaves. Instrument Application Time Difference IRGA (Infrared Gas Analysis) CO₂Analysis module and dual laser tuning fast response water vapor sensor for precise measurement of CO on blade surface₂To investigate the parameters related to photosynthesis between leaves and plants based on changes in concentration and water content. Through artificial light sources CO₂The control unit and temperature control unit can accurately regulate environmental conditions simultaneously, thereby measuring light intensity CO₂The effects of concentration and temperature on plant photosynthetic system. This instrument can be used in harsh environments such as high humidity and high dust, and has a wide range of applicability.

The left side of the picture shows the complete set of accessories and portable case for the photosynthesis instrument host. The picture shows the photosynthesis instrument host and handle. The right side of the picture shows the operator conducting a field experiment

application area

lResearch on Plant Photosynthetic Physiology

lResearch on Plant Stress Resistance

lResearch on Carbon Source and Carbon Sink

lThe response and mechanism of plants to global climate change

lScreening of new crop varieties

FEATURES

lEquipped with a handheld chlorophyll fluorescence analyzer and built-in with all common chlorophyll fluorescence analysis experimental programs, including two sets of fluorescence quenching analysis programs, three sets of light response curve programs, OJIP test, etc

l

Color LCD touch screen, both the screen and control unit adopt film sealing technology, which can be used in high humidity and dusty environments

lChoose either white light or RGB (Red Gree Blue) light source

lBuilt in GPS module, accurately obtaining latitude, longitude, and altitude data

lFully automatic and independent control of environmental parameters (air humidity, CO)₂Concentration, temperature, light intensity)

lAccurate measurement of CO₂And water vapor data

lPortable design, lightweight and small in size, weighing only 4.1Kg

lErgonomic design, comfortable shoulder strap, easy to carry and operate

lThe handle is equipped with a miniature IRGA, effectively reducing CO₂Time

lCan operate in harsh environments, sturdy and durable

lEasy interchangeability of different types of leaf chambers and leaf clamps

lCarefully selected leaf chamber materials ensure CO₂And moisture measurement accuracy

lLarge data storage capacity, using a plug and unplug SD card

lEasy to maintain, all areas of the leaf chamber are easy to clean

lAdopting low-energy technology, the outdoor single battery has a long continuous working time, up to 16 hours

lReal time graphic display function

The above picture shows M. from the Department of Plant Sciences, University of Cambridge, UK Dr. Davey's work images on algae photosynthesis research in Antarctica were selected as the first choice due to the lightweight, compact, durable, and long-lasting characteristics of the LC series photosynthetic apparatus.

TECHNICAL INDEX

lMeasurement parameters: photosynthetic rate, transpiration rate, intercellular CO₂Concentration, stomatal conductance, leaf temperature, leaf chamber temperature, photosynthetically active radiation, air pressure, GPS data, etc., can be used for light response curve and CO analysis₂Response curve measurement.

lHandheld chlorophyll fluorescence analyzer (optional)

1. The measurement parameters include F₀、 Ft、Fm、Fm’、QY_Ln、QY_Dn、NPQ、Qp、Rfd、RAR、Area、M₀、 More than 50 chlorophyll fluorescence parameters, including Sm, PI, ABS/RC, as well as light response curves for 3 light application programs, 2 fluorescence quenching curves, OJIP curves, etc

2. High time resolution, up to 100000 times per second, automatically drawing OJIP curves and providing 26 OJIP test measurement parameters including F₀、 Fj、Fi、Fm、Fv、Vj、Vi、Fm/F₀、 Fv/F₀、 Fv/Fm、M₀、 Area、Fix Area、Sm、Ss、N、Phi_P₀、 Psi_₀、 Phi_E₀、 Phi-D₀、 Phi_Pav、PI_Abs、ABS/RC、TR₀/RC ET₀/RC DI₀/RC and others

lCO₂Measurement range: 0-3000ppm

lCO₂Measurement resolution: 1ppm

lCO₂Using infrared analysis, differential open circuit measurement system, automatic zeroing, automatic pressure and temperature compensation

lH₂OMeasurement range: 0-75mbar

lH₂OMeasurement resolution: 0.1mbar

lPARMeasurement range: 0-3000 μ mol m^-2s^-1Cosine correction

lLeaf chamber temperature: -5-50 ℃ Accuracy: ± 0.2 ℃

lLeaf temperature:- 5 - 50℃

lAir pump flow rate: 100-500ml/min

lCO₂Control: Controlled by internal CO₂Supply system provides up to 2000ppm

lH₂OControl: can be higher or lower than environmental conditions

lTemperature control: controlled by micro Peltier components, with ambient temperature ranging from -10 ℃ to+15 ℃, and all leaf chambers automatically adjustable

lPARControl: RGB light source maximum 2400 μ mol m^-2s^-1， LED white light source maximum 2500 μ mol m^-2s^-1

lMultiple controllable temperature chambers and blade clamps with light sources can be optionally selected

1. Wide leaf chamber: length x width of 2.5 x 2.5cm, suitable for broad-leaved and most leaf types

2. Narrow leaf chamber: length x width of 5.8 x 1cm, suitable for strip leaves with a width less than 1cm

3. Coniferous leaf chamber: approximately 69mm in length and 47mm in diameter, suitable for clustered needles (white light source)

4. Small leaf chamber: The diameter of the leaf chamber is 16.5mm, and the measured area is 2.16cm²

5. Soil respiration/small plant chamber: measuring soil respiration or photosynthesis of whole herbaceous plants below 55mm in height, with a bottom diameter of 11cm

6. Multi functional measurement room: 15 × 15 × 7cm in length, width, and height, divided into upper and lower parts. The upper part measures photosynthesis of small plants, while the lower part measures soil respiration

7. Fruit measurement room: consisting of two parts, the upper part is transparent and the lower part is made of metal. It can measure a maximum diameter of 11cm and a maximum height of 10.5cm for fruits

8. Canopy measurement room: bottom diameter 12.7cm, height 12.2cm, suitable for surface canopy

9. Fluorescence analyzer adapter: suitable for connecting multiple chlorophyll fluorescence analyzers

The above diagram, from left to right, shows the wide leaf chamber, narrow leaf chamber, LED light source, fluorescence analyzer combined leaf chamber, and small leaf chamber

The above figure shows from left to right the coniferous chamber, fruit measurement chamber, soil respiration chamber, multifunctional measurement chamber, and canopy chamber

lDisplay: Color WQVGA LCD touch screen, 480 x 272 pixels, size 95 x 53.9 mm, diagonal length 109mm

lData storage: SD card, compatible with a maximum capacity of 32GB

lData output: Mini-B USB interface, RS232 nine pin D-type interface, maximum 230400 baud rate PC communication

lPower supply system: Built in 12V 7.5AH lithium-ion battery, can work continuously for up to 16 hours, intelligent charger

lSize: Host 230 × 110 × 170mm, measuring handle 300 × 80 × 75mm

lWeight: Host 4.1Kg, measuring handle 0.8Kg

lOperating environment: 5 to 45 ℃

Typical Application 1

Glyphosate reduces shoot concentrations of mineral nutrients in glyphosate-resistant soybeans, Zobiole L. et al. 2010, Plant and Soil, 328(1): 57-69

This study treated different types of glyphosate resistant soybeans with glyphosate and found that various photosynthetic parameters of soybeans, including chlorophyll content, stomatal conductance, photosynthetic rate, and transpiration rate, were reduced.

Typical Application 2

Methanol as a signal triggering isoprenoid emissions and photosynthetic performance inQuercus ilex, Seco R. et al. 2011, Acta Physiologiae Plantarum, 33(6): 2413-2422

The left side of the figure shows the chamber device designed in this study to investigate the physiological changes of Quercus ilex when cutting off some leaves (simulating gnawing) and adding methanol (simulating the signal released when other nearby plants are gnawed). The right side of the figure indicates that both treatments increased the net photosynthetic rate of the plants.

origin

Britain

Optional technical solutions

1) Composition of photosynthesis and chlorophyll fluorescence measurement system with chlorophyll fluorescence meter

2) Combined with FluorCam to form a photosynthesis and chlorophyll fluorescence imaging measurement system

3) Optional hyperspectral imaging for studying the spatiotemporal changes of photosynthesis from single leaf to composite canopy

4) Optional option O₂Measurement unit

5) Optional infrared thermal imaging unit for analyzing the dynamic conductivity of pores

6) Optional PSI intelligent LED light source

7) Optional handheld plant (leaf) measurement instruments such as FluorPen, SpectraPen, PlantPen, etc. can be used to comprehensively analyze the physiological and ecological characteristics of plant leaves

8) Optional ECODRONE ® Unmanned aerial vehicle platform equipped with hyperspectral and infrared thermal imaging sensors for spatiotemporal pattern investigation and research

References (only list some representative references)

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3. Alvarado-Sanabria,O., Garcés-Varón, G. and Restrepo-Díaz, H. (2017). Physiological Response of Rice Seedlings (Oryza sativa L.) Subjected to Different Periods of Two Night Temperatures. Journal of Stress Physiology & Biochemistry, Vol. 13, No. 1, 2017, pp. 35-43. ISSN 1997-0838.

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14. P Zou, X Lu, C Jing, Y Yuan, Y Lu, C Zhang (2018). Low-Molecular-Weightt Polysaccharides From Pyropia yezoensis Enhance Tolerance of Wheat Seedlings (Triticum aestivum L.) to Salt Stress (2018 Frontiers in plant”, 2018 – frontiersin.org

15. MEB Brito, LAA Soares, WS Soares Filho (2018). Emergence and morphophysiology of Sunki mandarin and other citrus genotypes seedlings under saline stress (2018)- Spanish Journal 2018 – revistas.inia.es

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17. X Zhang, Y Fan, Y Jia, N Cui, L Zhao (2018). Effect of water deficit on photosynthetic characteristics, yield and water use efficiency in Shiranui citrus under drip irrigation (2018- Transactions of the, 2018 – ingentaconnect.com

18. JES Ribeiro, AJS Barbosa, SF Lopes (2018). Seasonal variation in gas exchange by plants of Erythroxylum simonis Plowman (2018)- Acta Botanica”, 2018 – SciELO Brasil

19. TB de Oliveira, L de Azevedo Peixoto, PE Teodoro (2018). The number of measurements needed to obtain high reliability for traits related to enzymatic activities and photosynthetic compounds in soybean plants (2018)- PloS one, 2018 – journals.plos.org

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21. W Zhang, XX Chen, YM Liu, DY Liu, YF Du (2018). The role of phosphorus supply in maximizing the leaf area, photosynthetic rate, coordinated to grain yield of summer maize (2018)- Field Crops ”, 2018 ØC Elsevier

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