LR WhiteResin embedding agent is an aromatic cross-linked acrylic acid with high hydrophilicity. It was previously only used for electron microscopy immunohistochemistry, but now it is also applied in optical microscopy. Because the polymerized resin is hydrophilic, it is easy for the resin to penetrate into the slices in immunohistochemistry experiments, and the slices do not need to undergo freeze etching (etching often affects tissue antigens) treatment. Discovered in practiceLR WhiteIt has good stability, low dehydration requirements, short polymerization time, easy slicing, and short staining time.

LR WhiteLow resin viscosity（8cps）Non toxic, suitable for sample production in optical and electron microscopes. Many researchers have experienced the difficulty of embedding plant tissue with other embedding resinsLR Whiteresin,You will have a better embedding experience!

LR WhiteThe resin is currently hard, medium, and soft3For most researchers, hard and intermediate products are preferred for products of different grades. The products currently being sold are not ready to use（prefixed form-ready to use）But it's a non catalytic version（non-catalyzed version）: Resin+Catalyst（500g resin+9.9g Benzoyl Peroxide）. useLR WhiteBefore resin polymerization embedding, the catalyst is proportionally added to the resin at room temperature, and immediately shaken well to fully dissolve it. This process generally requires24Do not attempt to accelerate the dissolution of the catalyst to heat it up during the hour! The resin that has been fully dissolved and mixed must be returned4℃ is used to ensure its shelf life. Generally, mixed resins can be stored carefully for six months to a year (as stated in this book, it is recommended to use them up quickly!).

LR WhiteIt is a bipolar polyhydroxylated acrylic resin with four different polymerization methods:1.Thermal polymerization:60-65℃, approximately time48Hour. Some ovens cannot control the polymerization temperature so accurately, and if the embedding block is too fragile, temperature is the first parameter that needs to be checked.2.UV polymerization（365nm）The recommended temperature for general UV polymerization is0℃ and-20Between ℃, the time required is approximately24For hours, it is recommended to use a low-temperature UV curing box.3.Low temperature chemical polymerization: adding acceleratorsacceleratorAdd the accelerator mixture to the resin (note: this accelerator needs to be purchased separately)1Aggregation within an hour, generally10-20Due to the minute, the permeability of the mixture is slightly lower. The polymerization process is an exothermic reaction, and it is generally recommended to use an ice water mixture to cool down and embed the mold.4.Microwave polymerizationMicrowaveAttention should be paid to the temperature of the water used for microwave polymerization according to the instructions.

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