Low viscosity low-temperature oil is widely used in crystallography - mainly as a low-temperature protective agent during sample flash freezing, to prevent sample dehydration, solvent evaporation, exposure to air, and oxidation above room temperature. The diffraction pattern of frozen protein crystals shows an ice ring(This mainly occurs due to the crystallization phenomenon around the crystal nucleus in aqueous solution, rather than the crystallization phenomenon from internal solvents)Low temperature oil is a substitute for this solution that surrounds crystal nuclei, although it has minimal damage, it should be attributed to osmotic shock.

along withXThe progress of radiation sources, optical technology, detection equipment, and sample adhesion technology has led to the formation of protein crystals as small as5umIt can be used for structural inspection. roughly50umThe crystals quickly dehydrate and shrink in air, and can shrink to within seconds10umThe following crystals.

The significant dehydration phenomenon that occurs during the process of removing the solution around the crystal for simultaneous crystal flash storage poses a threat to the crystal. To minimize this hazard, we can transfer the crystals coated with low-temperature oil toMicroMountperhapsMicroMeshAdd another drop of low-temperature oil when going up(useMicroSieves ™tool).

However, the low-temperature oil surrounding the crystal isXRadiation scattering reduces the signal-to-noise ratio of diffraction, while a large amount of thermal storage leads to a decrease in flash freezing rate, increasing the demand for permeable antifreeze agents. This problem becomes more pronounced in smaller crystals. When we apply mineral oilParatone oilAs well as other high viscosity oils, some of the volume around the crystal may exceed ten times the volume of the crystal, which may cause difficulties in sample movement. For long rod-shaped or sheet-like crystals, the presence of these oils during flash freezing may cause crystal brittleness or an increase in crystal pattern bandwidth.

LV CryoOil ™Low viscosity low-temperature protective oil has the lowest viscosity among all consistent fluorinated polymer oils(Paratone oilEquivalent in viscosity to other vegetable oils, andLV CryoOil ™The viscosity is equivalent to its1/10)Usually, the crystal is immersed in this oil, and then lifted gently to shake off excess oil, to obtain approximately10umThick blanket.LV CryoOil ™Oil can be applied under vacuum, it has very low air pressure, excellent chemical inertness, and excellent thermal stability.

Ordering information: