FastDNA ® Spin Kit for Soil can quickly and effectively extract genomic DNA from soil and other environmental samples within 30 minutes. This kit is specifically designed for FastPrep ® The instrument design can directly extract genomic DNA from the spores and spores of all organisms in the soil, including bacteria, yeast, fungi, algae, nematodes, and even true bacteria. And it can also extract DNA from other environmental samples, such as sediments, excreta, wastewater, snow water, etc. Only 500mg of sample is needed to obtain sufficient DNA. The experimental principle is to treat the sample with a special cracking medium E, and then use a spin filter based on the principle of silicon adsorption of DNA in GENECLEAN ® The purified DNA can be directly applied to PCR, restriction enzyme digestion, electrophoresis, and other downstream experiments.

Product Features:

1. Cooperate with FastPrep ® Instrument cracking sample processing is faster, has better repeatability, and higher yield;

2. Suitable for isolating genomic DNA from microorganisms and animal and plant tissues of different types of soil;

3. For samples from different sources, DNA can be lysed and separated within 60 minutes, combined with FastPrep ® The instrument only takes 30 minutes;

4. Does not contain toxic or harmful reagent components.

Product application:

Soil samples, fecal samples, environmental water samples, wastewater samples, sediment samples, and other environmental samples.

Technical advantages:

1. It can effectively handle some difficult components in soil samples, such as eubacterial spores, endospores, gram positive bacteria, yeast, nematodes, algae, fungi, etc.

The MT Buffer and Sodium Phosphate Buffer used during sample processing can effectively protect nucleic acids throughout the extraction process and minimize RNA contamination.

3. Purification method based on silicon beads can effectively remove PCR inhibitors such as humic acid and polyphenols

The purified DNA can be directly used in subsequent experiments such as PCR and enzyme digestion.

5. For samples with particularly high humic acid content, additional processing methods should be applied.

Storage conditions:

The reagent kit should be stored at room temperature (15-30 ℃).

Specifications: