PhenoTron-SRPlant Phenotype Imaging Analysis System

Plant Phenotyping from Shoots to Roots

PhenoTron-SRThe plant phenotype imaging analysis system can simultaneously perform phenotype imaging analysis on crop roots, seedlings, and crop canopies. The system consists of a host system and a spectral imaging system: the host system includes a system platform (host box), a control unit, a sample holder, a data processing server, etc; The spectral imaging system consists of a spectral imaging unit (including imaging sensors, light sources, gimbals, etc.) and an automatic scanning axis. Its main technical features are as follows:

1) Phenotyping from shoot to root:Phenotypic imaging analysis can be performed on the aboveground part (shoot) and underground root system (RhizoBox, a specially designed RhizoTron plant root observation culture box) of plants

2) The standard configuration includes 60 degree tilt automatic scanning imaging, as well as hyperspectral imaging analysis and RGB imaging analysis of RhizoBox roots and seedlings. Other angles such as 45 degrees, 70 degrees, and 90 degrees (vertical scanning imaging) are optional

3) Optional top canopy RGB imaging analysis, infrared thermal imaging analysis, hyperspectral imaging analysis, chlorophyll fluorescence imaging analysis (optional sample tray suitable for normal culture pots)

4) Optional iPOT digital plant culture pot or RhizoBox root culture box for continuous monitoring of soil moisture temperature, weight, plant growth, photosynthetic efficiency, etc PI（performance Index）、 Physiological and ecological indicators such as stem flow can automatically collect soil seepage water and perform soil nutrient analysis

5) Modular structure with powerful system expansion capabilities, automatic universal casters on the system platform for easy mobility

6) Remote control, automatic operation, data collection and storage, and other functions are available

From left to right are: system perspective view, internal structure diagram of the system (including side tilted automatic scanning axis, RhizoBox, top imaging sensor, etc.), RGB imaging of cotton roots, and hyperspectral imaging analysis of cotton roots (900-1700nm)

Technical indicators:

1) The control unit is an embedded operating system with a fully Chinese touch screen, which facilitates system debugging, trial operation, etc

2) Users can remotely control the camera and platform through PC based full Chinese GUI software

3) Support combination commands: up to 10 commands can be set to achieve unmanned operation

4) Serial communication and TCP/IP protocol are used to achieve communication control and remote communication protocol with microcontrollers

5) Equipped with an automatic scanning axis tilted at 60 degrees as standard, it can prevent soil disturbance caused by tilted root culture boxes

6) Automatic scanning axis push scanning speed and accuracy: adjustable from 1-90mm/s, with a movement accuracy of 1mm

7) Effective scanning range: standard 120cm

8) Sample holder: Standard RhizoBox root box 40cm wide (adjustable width, 40cm is the maximum standard width), maximum height up to 100cm, optional with other specifications

9) The sample tray is suitable for a single large culture pot or multiple small culture pots, and can be customized according to customer needs

10) VNIRHyperspectral imaging:

a) Band range: 400-1000nm

b) Number of bands: 224 channels

c) Spectral resolution: FWHM 5.5nm

d) Spatial resolution: not less than 1024x1024

e) Signal to Noise Ratio 600:1

f) Analysis parameters:Imagery measurement and analysis of crop biochemical and physiological indicators such as chlorophyll content, anthocyanin content, carotenoid content, light use efficiency, health index, coverage, stress, and more than 20 other parameters

11) SWIRNear infrared hyperspectral imaging:

a) Band range: 900-1700nm

b) Number of bands: 224 channels

c) Spectral resolution: FWHM 8nm

d) Spatial resolution: not less than 640x640nm

e) Signal to noise ratio: 1000:1

f) Analysis parameters: Imaging measurement analysis of NDNI normalized N index, NDWI normalized water index, MSI water stress index, etc

12) Infrared thermal imaging (optional):

a) Resolution: 640x512 pixels

b) Measurement temperature range:-25°C－150°C

c) Sensitivity: 0.03 ° C (30mK) @ 30 ° C

d) Spectral range:7.5－13.5μm

e) Sensor: Uncooled infrared focal plane sensor, calibrated at multiple points (with calibration certificate)

f) 1-142x digital zoom

g) The software has a color palette (14 color combinations including natural, rainbow, grayscale, gradient, etc.), difference technology, temperature range setting (to change color distribution or highlight selection range, etc.), isotherm mode, selection analysis (points, lines, polygons, etc.), temperature scanning (to display the temperature distribution curve of the selected line, etc.), profile temperature, time chart, etc; Can display image information; Having reporting modes, etc; Control settings can be made

13) RGBColor imaging:High resolutionRGBImaging, resolution up to18MPixels，10 Multiple optical zoom, optional with other resolution lenses, equipped with professional plant root and shoot analysis software

14) Chlorophyll fluorescence imaging unit (optional):

a) Professional high-sensitivity chlorophyll fluorescence imaging CCD, frame rate 50fps, resolution 720x560 pixels, pixel size 8.6x8.3µm

b) Photochemical light up to 1000µmol.m^-2. s^-1Adjustable, saturated pulse 3900µmol.m^-2. s^-1

c) Can automatically run protocols such as Fv/Fm, Kautsky induction effect, fluorescence quenching analysis, light response curve, etc

d) 50Multiple chlorophyll fluorescence automatic measurement and analysis parameters, including Fv/Fm, Fv '/Fm', Y (II), NPQ, qN, qP, Rfd, ETR, etc., automatically generate chlorophyll fluorescence parameter maps

e) Automatically synchronize the display of chlorophyll fluorescence parameters and parameter graphs, chlorophyll fluorescence dynamic curves, and chlorophyll fluorescence parameter frequency histograms

15) Optional air temperature and humidity, lighting, and top laser ranging module

16) System platform specifications: standard configuration of approximately 120cm x 60cm x 160cm (length x width x height), weight of approximately 50kg

The left image shows the top canopy hyperspectral imaging (NDVI), and the right image shows the extraction of root imaging (provided by Ecolab Laboratory of Yiketai Ecological Technology Company)