This product uses oxygen sensing technology to detect the oxygen consumption during seed germination to identify the vitality level of seeds. It is from the NetherlandsASTEC GlobalcompanyDeveloped based on the principle of fluorescence quenching. As shown in the picture1As shown, the oxygen sensing monitor uses blue light as excitation light and emits it into a closed test tube (seed germination container)
Releasing blue light, the blue light shines on the fluorescent substance and excites red light, while oxygen molecules can reduce the energy of red light (i.e. quenching effect)
The increase in oxygen pressure leads to a decrease in both fluorescence intensity and the lifetime of fluorescence pulses. Therefore, the time and intensity of exciting red light are related to the oxygen molecules
The concentration is inversely proportional. the NetherlandsASTEC GlobalThe fluorescent dye used by the company is a metal organic dye, and fluorescence life cycle technology is used to determine fluorescence
The attenuation of intensity is sensed by optical fibers to a computer, thereby determining the oxygen content. The calculation formula is:T=F([O2])=F(I/I0),Among them,I、I0
The fluorescence intensity of the samples before and after germination, respectively,TFor the fluorescence lifetime,[O2]For oxygen concentration.